Which chemicals are used during the Gram Stain procedure?

4 chemicals

Explanation:

There are mainly 4 steps of gram staining.

1- crystal violet stain drained on the heat fixed slide.

2- then wash the slide and use iodine.

3- then wash the slide with water and use acetone as a decolorizer.

4- then use safranine as a counter stain.

So there are a total of 4 chemicals used named crystal violet, iodine, acetone, and safranine.

a. by using fur or clothes to trap heat energy near the body

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.