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Biology, 07.04.2020 02:38 ammerismccormick52
Different cloning vectors are able to accept inserts of different sizes. Small plasmid vectors, for example, can clone insert fragments up to about 20,000 bp long. The Human Genome Project scientists also used large plasmid vectors base pairs long. Which of the following statements is true about g call Bacterial Artficial Chromosomes (BACs) that could clone fragments of the human genome 100.000-200,000 enomic libraries made in small plasmids vs. BAC vectors? a. A complete genomic library made in small plasmid vectors will not contain overlapping recombinant clones; a complete genomic library made in BAC vectors will contain overlapping clones and this is necessary regardless of insert size. b. A complete genomic library made in small plasmid vectors will contain more independent recombinant clones than a complete genomic library made in BAC vectors. c. lt is impossible to generate a complete genomic library in small plasmid vectors because it would require more genomic DNA fragments than it is possible to obtain.
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