Assuming that the double stranded DNA template for digestion has a GC content of 50%, what is the average distance between restriction sites for a restriction endonuclease with a recognition motif of ACGT

Answer and Explanation:

The average distance between restriction sites refers to how frequently the restriction endonuclease with the recognition sequence ACGT might cut the double-stranded DNA.

We know that the frequency of cutting in a determined DNA sequence is (1/4)ⁿ, n being the number of bases in the recognition sequence, and 4 being the number of possible nucleotides.

So, in the present example, the restriction endonuclease recognizes the sequence ACGT, formed by 4 bases. This is, n=4.

The frequency of the ACGT sequence is (1/4)⁴= 1/256. The enzyme cuts every 256 bases. The average distance between restriction sites 0.25 kb.

Note: This is for only one of the strands of DNA.    

the transfer for conduction is the heat transfering from the liquid to the spoon

the transfer for convection is the heat rising from the liquid to the air

the transfer for radiation is the heat going through the sides of the cup and to the air

at least that is what i would do