Arrange the following sequence of procedures in the proper order in this experiment done to determine proteins present in pea plants during germination. 1. Load the proteins in polyacrylamide gel electrophoresis and separate them 2. Break the cells by grinding them in liquid nitrogen and suspending in a buffer 3. Select a suitable tissue to isolate proteins expressed only during germination 4. Centrifuge the broken cells to purify proteins from other molecules

The options:

A) 1,3,2,4

B) 3,2,1,4

C) 2,4,1,3

D) 3,2,4,1

E) 2,4,3,1

The CORRECT ANSWER IS D)

D) 3,2,4,1

Explanation:

At the initial stage, it is needed to determine the area you need the cells from.

This process is then accompanied with disruption of the cells to have access to the proteins

Next course of action is to centrifuge the constituents in order to separate the proteins.

At the final phase, it's important to fill up the gel with the proteins coupled with running to aid easy isolation of the constituents based on size and charge.

ecological efficiency: % of usable energy transferred as

biomass from one trophic level to the next typical is 10% efficient (90% of

energy is lost),is diagramed as the pyramid of energy flow. then osprey eagle

are tertiary consumer and the shrimp are primary consumer, so to balance

mass of shrimp (1/0.1) / 0.1 = 100 kg