Krebs and colleagues identified the intermediates in a series of experiments where they took short chain acids known to be in the cell and added them to enzyme extracts from eukaryotic sources. How would they know if a given acid was an intermediate?

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cellular respiration oxidizes food into energy in the form of atps.

glycolysis (6 c hexose) > 2x atps are used > 6c hexose biphosphate > 2 x 3c triphosphate >

2 x 3c pyruvate (2x nadh produced from nad) and (4x atp produced)

pyruvate actively pumped mitochondrial matrix.