Chloroplasts were isolated from spinach leaves and incubated in an acid medium along with ADP, radioactive PO4 (Pi), and nitrofluorene, a herbicide that inhibits the photosynthetic electron transport chain. The chloroplasts were then transferred to the dark, and the amount of radioactive ATP was then measured. What would you expect to happen to ATP production in this system?

a. ATP would not be produced because the herbicide will shut off AIP synthase.
b. ATP would not he produced, because this experiment is carried in the dark.
c. No ATP would be produced because the pH in the interior of the thylakolds would be too low.
d. ATP would not be produced because no proton gradient would be fumed.
e. Abundant ATP would be produced but NADPH would not.
f. A lot or ATP would be produced because shutting down the electron transport chain stimulates photophosphorylation.

ATP would not be produced because no proton gradient would be formed. The correct option is D.

Explanation:

Chloroplast contains chlorophyll which are the photosynthetic molecules used by plants(energy producers) to trap in light energy from the sun. When isolated and incubated with a herbicide that inhibits the photosynthetic electron transport chain,ATP would not be produced because no proton gradient would be formed. This is so because electron transport chain lead to the generation of ATP in the light-dependent reactions of photosynthesis through the movement of electrons down the electron transport chain which drives the formation of proton gradient that is used to make ATP. I hope this helps, thanks.

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