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Biology, 23.04.2020 20:14 darkshaders11

Genes and Genomes a. You start an experiment with a yeast strain that is an adenine and methionine auxotroph, subject it to UV mutagenesis, and isolate a mutant that is incapable of growth at 16'C (yeast normally grow at 30°C but can also grow at a wide range above and below this standard temperature).

i. To identify the gene that has been mutated, you take the wild type yeast genomic DNA, break it into pieces, and introduce these pieces into the mutant cells. Which of the following observations (on its own) will let you conclude that the mutant picked up the wild type copy of the UV-mutated gene? Select all that apply.

A. It grows at 16 C
B. It grows at 30°C
C. It grows without adenine in the environment
D. It grows without methionine in the environment

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