C. (1) A new form of Cry1A protein has been developed that targets the mutated cadherin protein and confers resistance to mutant rootworm genotypes. Order the following steps, (1 through 5) to introduce the gene for the new Cry1A protein into an elite corn hybrid. Answer using numbers 1, 2, 3, 4, 5 Test for expression of the gene in transgenic plants Regenerate plants carrying the new gene by tissue culture Create a construct carrying the gene using recombinant DNA technologies Transform the construct carrying the gene into corn plant cells Move the gene into high-performing varieties

1) Create a construct carrying the gene using recombinant DNA technologies.

2) Transform the construct carrying the gene into corn plant cells.

3) Regenerate plants carrying the new gene by tissue culture.

4) Test for expression of the gene in transgenic plants.

5) Move the gene into high-performing varieties by conventional breeding.

Explanation:

volcanoes (an event in the geosphere) release a large amount of particulate matter into the atmosphere. these particles serve as nuclei for the formation of water droplets (hydrosphere). rainfall (hydrosphere) often increases following an eruption, stimulating plant growth (biosphere).

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