The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 6 repeats in your product, what was the size of your final PCR product?

The size of your final PCR product will be ; 214

Given that the new primer used amplifies 130 base pairs that are adjacent to

a) 14 base pair  and

b) Has 6 repeats

Determine the size of the final PCR product

( number of base pair * number of repeats ) + Initial/primer amplicon value

= ( 14 * 6 ) + 130

= 84 + 130

= 214

Hence we can conclude that the size of the final PCR product is 214

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The answer is 214.

Explanation:

This is because 130 base pair is adjacent to 14 base pair repeat , and you have 6 repeats. So 14 multiplied by 6 repeats is 84. If you add this to your primer amplicon, you will get the size of final PCR product i.e.. 214.

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