How do the chromosomes line up in Metaphase l?

In the third step of mitosis, called metaphase, each chromosome lines up in a single file line at the center of the cell. By this point in time, the membrane enclosing the nucleus has dissolved, and mitotic spindles have attached themselves to each chromatid in all the chromosomes.

many other transport proteins, often called pumps, move materials against a concentration gradient. active transport drives molecules across a membrane from a region of lower concentration to a region of higher concentration. this process, shown in figure 5.1, uses transport proteins powered by chemi- cal energy.