A. Describe how the structure of a prokaryotic chromosome differs from the structure of a eukaryotic chromosome.

B. Explain why DNA replication cannot proceed to the regions of the chromosome unless topoisomerase binds ahead of each advancing replication fork.

C. Use the template of a replication fork to draw arrows that represent both continuous and discontinuous DNA synthesis. Draw one long arrow to show continuous DNA synthesis and three arrows to show discontinuous DNA synthesis. The arrows should point in the direction of nucleotide addition, and the three arrows showing discontinuous synthesis should be numbered 1, 2, and 3, in the order of fragment synthesis.

D. Explain why any newly synthesized strand of DNA is the result of both continuous and discontinuous DNA synthesis.