(APPLY YOUR KNOWLEDGE)
in 1951, a chemical company that manufactured fertilizer began dumping its waste in dinamata Bay.
apan The waste contained mercury that contaminated the ecosystem, making over 2.200 people
with mercury D oning Citizens suffered from high fever convulsions, psychosis, loss of consciousness
coma, and many times ending in death. Approximately 900 people died, as well as most of the birds and
somesticated animals in the area. Scientists investigating the tragedy concluded that people and an mas eating the fish had ingested mercury, which was causing the poisoning and sickness. After 12 years, the dumping was stopped

A. as elemental mercury moves through an aquatic ecosystem, it takes different chemical forms to
become methylmercury, which can then move through food chains. Explain the sequence of events
that take place.

B. Methylmercury is a persistent neurotoxin. Explain what this means

C. Explain why humans and other animals who ate the fish from Minamata Bay suffered from mercury
poisoning.

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

Spring tides, when the high tides are at their highest and low tides at their lowest. what is it about these positions that causes these high and low tides?

What causes darkening of the skin as melanin production increases