You hypothesize that a transcriptional regulator may control expression of specific genes in LB medium, and want to find out what these genes are. You have made a set of strains (i. e., library) in which a promoterless lacZ was inserted at varied chromosomal locations. Design an experiment to identify these genes using agar plates with LB and X-gal. Describe the steps in this procedure.

if the promoterless lacZ is inserted close to any gene(s) or operon, then the laz gene will also be transcribed when the operon gets expressed. The polycystronic RNA will eventually produce a beta-galactosidase protein(product of lacZ gene) which will cleave X-Gal( structural analog to lactose), which will produce blue chromogenic substance.

prepare LB agar. Allow it to cool, add X-gal before it solidifies, pour to plates.

create grids and patch plate each strain on it. Just pick the strain that looks blue, amplify the sequence upstream of the lacZ and sequence it.

Explanation:

Just pick the strain that looks blue, amplify the sequence upstream of the lacZ, and sequence it.

the endocrine system secretes hormones into blood and other body fluids. these chemicals are important for metabolism, growth, water and mineral balance, and the response to stress. hormones provide feedback to the brain to affect neural processing. reproductive hormones affect the development of the nervous system.

lysosome is this part of the cell is surrounded by a very thick outer membrane to protect the rest of the cell from its strong enzymes.