Determine the nature of the expression of the lacZYA genes, represented by L, and label each genotype as constitutive, inducible, or no transcription in the presence of lactose. Assume that all promoters are wild type. Symbol Representation I− lacI mutant cannot bind to the operator IS lacI mutant always binds to the operator OC Operator mutant that prevents repressor binding F' the wild‑type operator, O+, and lacI gene on a plasmid L lacZYA genes

Explanation:

Symbol          Representation

I-                     laci mutant cannot bind to the operator

Is                    laci mutant always bind to the operator

OC                 Operator mutant that prevent repressors binding

F’                    the wild type operator, O+, and laci gene on a plasmid

L                     lacZYA genes

I+ O+ L+   Inducible

I+ OC L+   Constitutive

I+ OC L+, F’   Constitutive

I- O+ L+, F’   Inducible

Is O+ L+    no transcription

Is O+ L+, F’   no transcription

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