) After decades of work a scientist isolated a small amount of attractase, an enzyme producing a powerful human pheromone. To produce attractase for his personal use in large quantities, he obtained a complete genomic clone of the attractase gene, connected it to a strong bacterial promoter on an expression plasmid into E. coli cells. He was devastated to find out that no attractase was produced in the cells. What is a likely explanation for the failure

A complete eukaryotic gene was used into the bacteria genome

Explanation:

The atractase gene is an eukaryotic gene, which means that is composed of a  promoter, introns and exons. When the gene was connected to a strong bacterial promoter only the exons should be used in order to promote the correct translation of the enzyme.

the cytokine-activated janus kinase (jak)-signal transducer and activator of transcription (stat) pathway has an important role in the control of immune responses. dysregulation of jak-stat signaling is associated with various immune disorders.

there are so many examples for that in different areas, like biology experiment carried out in our lab recently.

here's one link: