Suggest why explants are sterilised?line4

These tiny explants can be sterilised in dilute bleach to kill surface bacteria and fungi, then grown in a suitable culture medium. The explants divide by mitosis and grow but if the chemicals in the growth medium are correct, they will also start to differentiate into roots, shoots and leaves.

Explanation:

a neutral ph of 7 and normal organism temperature is needed for a certain enzyme to be effective.  

what would happen to the enzyme if the ph and/or temperature fluctuate greatly from the norm?  

a.the enzyme’s hydrogen bonds will break.  

b.the activation energy will increase.  

c.more product will be formed.

d.the ideal temperature and ph for the enzyme function will decrease.