1) The plasmid is 12 KB or 12,000 base pairs (kilo base pairs). If you use a restriction enzyme with one restriction site(cut) how many pieces of DNA do you end up with?
2) Explain the term “double digest”.
3) In the examples of double digests given, are all of the DNA fragments the same size? Why or why not?

the oxygenated blood then leaves the lungs through pulmonary veins, which return it to the left heart, completing the pulmonary cycle. this blood then enters the left atrium, which pumps it through the mitral valve into the left ventricle. from the left ventricle, the blood passes through the aortic valve to the aorta.

deoxygenated blood leaves through the right ventricle through the pulmonary artery. from the right atrium, the blood is pumped through the tricuspid valve (or right atrioventricular valve), into the right ventricle.

the titanic would be the correct answer