How are the blunt and sharp pipettes

used in the cloning process?

(please answer in simple words i don't even understand the question)

During the process of the type of cloning known as Somatic Cell Nuclear Transfer (SCNT), once the nucleus is removed from the egg cell, blunt and sharp pipette both are used. The purpose of the blunt pipette is to hold the egg cell in position whereas the sharp pipette penetrates the cell to take the nucleus out by sucking it - a process known as "enucleation". This nucleus is discarded. Rather, this nucleus is replaced by a nucleus taken out from a somatic cell (same way as described before) of the same organism.

Hope that answers the question, have a great day!  

the concept of nucleosome occupancy, which reflects the fraction of cells from the population in which a given region of dna is occupied by a histone octamer

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure