Why are the plasmid vector and EcoRI the appropriate choices for the creation of a recombinant plasmid containing the kanR gene?

Production of these sticky ends is another feature of restriction enzymes that makes them suitable for recombinant DNA technology. ... Hence, if both vector DNA and donor DNA are cut with EcoRI, the sticky ends of the vector can bond to the sticky ends of a donor fragment when the two are mixed.

enzyme active site and substrate specificity

enzymes bind with chemical reactants called substrates. there may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. in some reactions, a single-reactant substrate is broken down into multiple products