Biology, 04.08.2020 14:01 kanonhuffmanp3rnmy
You are doing an experiment in which you are using an in vitro translation system derived from rabbit reticulocytes, called a rabbit reticulocyte lysate, to translate mRNA in a test tube. The rabbit reticulocyte lysate contains ribosomes, translation initiation and elongation factors, tRNAs, aminoacyl tRNA synthetases, release factors, amino acids, and ATP. The lysate does not contain any mRNA, so you must add the mRNA you would like to translate to the system. A. When you analyze the proteins made from your lysate, you find that they are all only two amino acids long. At first, you think that there must be a mutation to a stop codon in the position of the third codon, so you do another experiment with a different mRNA. Again, all the newly synthesized proteins are only two amino acids long, so you conclude that there is a problem with some component in your lysate. Describe ONE possible problem in the translation system (lysate) that would result in the limitation of newly synthesized peptides to two amino acids in length.
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You are doing an experiment in which you are using an in vitro translation system derived from rabbi...
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