Which of the following valve closings can be auscultated during the "dupp" heart sound by placing the stethoscope at the left sternal border of the second intercostal space? A) Pulmonary semilunar
B) Right atrioventricular (tricuspid)
C) Left atrioventricular (mitral)
D) Aortic semilunar

Pulmonary semilunar

Explanation

Generally heart chambers are separated from each other by valves.Likewise blood vessels of the heart. When the heart contracts(systole) to ejects blood,theses valves close up,to prevent back flow and  the mixture of oxygenated with deoxygenated  blood. When these occurs the forces of closure leads   to certain distinctive sounds in the heart, detected by the stethoscope.Theses are called heart sounds,.

The pulmonary arteries take deoxygenated blood away from the heart to the pulmonary circulation in the lungs,while the aorta takes oxygenated blood away from the heart into the systemic circulation,The back flow of the blood into the pulmonary artery is prevented by the pulmonary  valves,while the aortic semi lunar valves  guide against this  in the aorta,The sounds produced by these closure is called  the dupp sound.,

However, because the the left sternal border of the second intercostal space is the location of the Pulmonary semiminular valves.Therefore the closure is the actual sound of dupp.

the last one: artificial selection/genetic engineering

plant breeding is modifying the genetic composition of the plant - but this isn't cloning (which is specifically introducing a gene into a genome). but you are artificially selecting for the phenotype that you want.

the second one is literally genetic engineering - the gene would have been cloned first and then inserted or genetically engineered into the new plant cell (the cotton).