Scientists have engineered bacteria to produce human proteins, such as factor VIII, a blood clotting factor used to treat hemophilia. Suppose the unmodified eukaryotic gene coding for factor VIII is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the factor VIII gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for factor VIII were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene. The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the factor VIII gene. The bacteria will not recognize the eukaryotic promoter to transcribe the gene. Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed.
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Scientists have engineered bacteria to produce human proteins, such as factor VIII, a blood clotting...
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