The scientist anneals a mutant oligonucleotide to the original M13 DNA. Then DNA polymerase replicates the M13 DNA. After DNA synthesis, the scientist adds a restriction enzyme, EcoRI, that recognizes the DNA sequence 5′−GAATTC−3′5′−GAATTC−3′ and cuts after the guanosine nucleotide. Following the restriction digest, the scientist adds exonuclease III for a short period of time. Another round of DNA polymerase‑catalyzed DNA synthesis completes the procedure. What is the nucleotide mixture required by the DNA polymerase reaction in this procedure?

What causes the force that pushes magma from the magma chamber through the pipe and vent of a volcano?

Match the types of competition to their descriptions

Where does all the water go? according to the environmental protection agency (epa), in a typical wetland environment, 39% of the water is outflow; 46% is seepage; 7% evaporates; and 8% remains as water volume in the ecosystem (reference: united states environmental protection agency case studies report 832-r-93-005). chloride compounds as residuals from residential areas are a problem for wetlands. suppose that in a particular wetland environment the following concentrations (mg/l) of chloride compounds were found: outflow, 60.4; seepage, 73.7; remaining due to evaporation, 26.4; in the water volume, 46.8. (a) compute the weighted average of chlorine compound concentration (mg/l) for this ecological system. (round your answer to one decimal place.) mg/l (b) suppose the epa has established an average chlorine compound concentration target of no more than 58 mg/l. does this wetlands system meet the target standard for chlorine compound concentration? yes. the average chlorine compound concentration (mg/l) is too high. yes. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is too high.