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You generate a Green Fluorescent Protein (GFP) tagged form of the Band 3 protein and express it in a red blood cell. The tag is on the extracellular side of the cell. You then bleach a small spot of the cell with a laser, removing the fluorescence from that spot. You already know that once ankryin and spectrin bind, they remain in association, as do Band 3 and ankryin. When you observe the spot you had bleached after 30 minutes, what do you expect? a. no green fluorescence b. green scattered throughout c. green fluorescence restricted to the outline of a triangle d. green fluorescence within a triangular domain e. not enough information to tell
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You generate a Green Fluorescent Protein (GFP) tagged form of the Band 3 protein and express it in a...
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