An IgG antibody that specifically recognizes a protein of interest, p50, was used to isolate p50 from a whole‑cell lysate. The resulting protein mixture can undergo SDS-PAGE to separate p50 from IgG. The molecular weight of p50 is 50 kDa,50 kDa, and the protein does not contain any cysteine residues. The molecular weight of IgG is 160 kDa.160 kDa. IgG contains two heavy chains and two light chains that weigh 50 kDa50 kDa and 25 kDa,25 kDa, respectively. Both types of chains contain cysteine residues. Select the components of the SDS‑PAGE loading buffer. a. bromophenol blueb. dithiothreitolc. glycerold. sodium dodecyl sulfatee. sodium chloride

An IgG antibody that specifically recognizes a protein of interest, p50, was used to isolate p50 from a whole‑cell lysate. The resulting protein mixture can undergo SDS-PAGE to separate p50 from IgG.

The molecular weight of p50 is 50 kDa,50 kDa, and the protein does not contain any cysteine residues. The molecular weight of IgG is 160 kDa.160 kDa. IgG contains two heavy chains and two light chains that weigh 50 kDa50 kDa and 25 kDa,25 kDa, respectively. Both types of chains contain cysteine residues.

here is the question

Select the components of the SDS‑PAGE loading buffer. (more than one)

bromophenol blue

dithiothreitol

glycerol

sodium dodecyl sulfate

sodium chloride

Explanation:

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.

when the displaced particles are perpendicular to the direction the wave travels, is called a transverse wave, whereas in longitudinal wave the displace practices are parallel to the direction of the wave.

in the given image:

first image shows - longitudinal wave

second image shows - transverse wave

third image shows - longitudinal wave

fourth image shows - longitudinal wave