Biology, 27.10.2020 17:40 vanessa23272

Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating a 3kb vector (25ng/μL) and a 2kb (10ng/μL) insert together, and the fragments have complementary sticky ends. Round final answers to the nearest hundredths place.

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