Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation, and the two experiments repeated, the measured max increases to 4.8 µM s−1, and the measured Km is now 15 µM.
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Further research shows that the purified happyase* used in the first two experiments was actually co...
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