You want to determine if gene X is a direct primary target of sigma S, or an indirect secondary target. To do so, you perform experiments with transcription and translation inhibitors and look for gene X RNA synthesis. Which experiment and result unambiguously points to gene X being a direct, primary target of sigma S

The question is incomplete,below is the complete question;

Regulons are collections of coordinately regulated genes and operons that can be controlled by alternating sigma factors such as sigma S,some of the genes and operons expressed in response to sigma S are primary targets that are directly bounded by sigma S,others are indirect, secondary targets of sigma S and are activated by a primary targets protein.

You want to determine if gene X is a direct primary target of sigma S, or an indirect secondary target. To do so, you perform experiments with transcription and translation inhibitors and look for gene X RNA synthesis. Which experiment and result unambiguously points to gene X being a direct, primary target of sigma S.

A) Experiment: transcription inhibitor Result: No gene X RNA production.

B) Experiment: translation inhibitor

Result: No gene X RNA production.

C) Experiment: transcription inhibitor

Result: gene X RNA production.

D) Experiment: translation inhibitor

Result: gene X RNA production.

The correct answer to the question is option A.

EXPERIMENT: TRANSCRIPTION INHIBITOR

RESULT: NO GENE X RNA PRODUCTION.

The protein that gives the specificity to RNA polymerase is known as a sigma factor. The specificity it gives is for RNA polymerase to bind at specific gene promoter sequence.

Here,we are looking at gene X RNA synthesis here by using transcription and translation as inhibitors.

Using inhibitor as transcription,there will not be transcription as well as translation occurance because translation occurs after the occurance of transcript (mRNA) synthesis through transcription.

However,if we decide to use inhibitor as translation,there will always be a chance for the synthesis of transcription that is gene X RNA production.