How would you produce a 10^1 dilution of a 3ml bacteria sample using the entire 3mL volume

Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.

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c. heat from the poles back down towards the equator.