Why is it important to perform a restriction digest after cloning your gene of interest fragments into the TOPO vector?

Restriction enzymes (restriction endonucleases) are proteins that cut DNA at (or close ... To be able to clone a DNA insert into a cloning or expression vector, both have ... you could carry out the two digestions simultaneously (double digestion)

Explanation:

the fossil record provides evidence of a common ancestor to many species.

we need additional information in order to determine the cause of death for anna garcia. there are at least 3 things with explanation included as to why we need them are as follows: