synthesis of an oligonucleotide with a sequence deviating in at least one base from the wild-type gene followed by replacement of the wild-type gene with the synthetic sequence is known a

Site-directed mutagenesis

Explanation:

Site-directed mutagenesis (SDM) is a technique widely used in molecular biology in order to generate site-specific, targeted changes in the DNA sequence of a gene of interest. The SDM protocol consists of using a complementary oligonucleotide (primer) that contains the desired mutation, which hybridizes to the target DNA sequence and thus can trigger the desired mutation. Nowadays, the versatile CRISPR-Cas9 genome editing system is the most used technique to produce targeted mutations in any gene of interest.

hello there are no statements below!

poor water quality affects most estuarine organisms, including commercially important fish and shellfish. the pollutants that have the greatest impact on the health of estuaries include toxic substances like chemicals and heavy metals, nutrient pollution (or eutrophication), and pathogens such as bacteria or viruses.