De IN In order to function efficiently, enzymes need to be at an ideal pH and temperature. Different enzymes have different ideal pH and temperature conditions. If the pH or temperature is extreme for a particular enzyme, it can even denature an enzyme, which can prevent it from binding and acting on its substrate. For the following two scenarios, name the variable (temperature or pH) that is affecting the function of the enzyme. TON A) ATP is produced by cellular respiration in your human body cells. There are a variety of enzymes th ATP, but one of those enzymes is called phosphofructokinase-1. This enzyme is sensitive to blood acid become more acidic if a patient is in respiratory distress.