An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem

The fish shown above is a tarpon. it is a fast-swimming and powerful open-water fish. its closest relatives, oddly, are burrow-dwelling conger eels that stay on the bottom. both eels and tarpon developed from snake-like larvae that float in the plankton during the first stages of life. once they mature, tarpon and eels are not found near one another in the ocean. the tarpon and the eel illustrate all of the following except