One type of mutation that can give rise to cleidocranial dysplasia in humans is an STR that causes an increased number of repeated Glutamine (Q, Glu) amino acids in the Runx2 protein that is produced by the RUNX2 gene. Below is a portion of the coding DNA strand for RUNX2, along with the amino acid sequence encoded. There is an STR triplet (CAG) repeated 22 times in the "wild type" allele, which results in a repeated sequence of Q amino acids. Your mission is to design a diagnostic assay for determining the approximate number of CAGs. 5' â GTG GCT GCG CAA (CAG) 22 GAG GCG GCG GCG â 3' N-term. VAAQQ22EAAA C-term. a. First, you need to design a PCR reaction to amplify just this portion of the gene from the two homologous chromosomes (the gene resides on Chromosome 6). What would be appropriate primer sequences to use for this PCR reaction? Forward primer: 5'- Reverse primer: 5'- b. Once you have amplified the DNA, what are the next steps you need to perform in order to visualize the PCR products? Make sure you mention the "controls" that must be included in your experiment to provide a meaningful interpretation of the results. Please use no more than 3 simple sentences! 2 NAME: c. Draw and/or describe (in THREE or fewer sentences) your predicted results for at least three types of cases, assuming that up to 22 CAGs causes wild-type phenotype, 4 additional CAGs over the normal 22 is predicted to cause mild CCD (even in heterozygotes), and more than this is associated with severe CCD (even in heterozygotes)?

i think that c)gold was found there

but i am not sure

picture a world where computers are building made-to-order personalized robotic arms and synthetic organs, drones are circulating above mars! due to the rapid growth of technology, the future has never been more unpredictable, and it presents a huge challenge in education: we are teaching students how to work in a future that we have never been unsure of. it is widely predicted that in eight years’ time, there will be more jobs in the fields of science, mathematics, technology and engineering than in any other discipline. and, as the technology continues to develop, it will soon become clear that addressing social problems will involve more innovative thinking, and analytical and technical knowledge than ever before. undeniably, there are no subjects that encourage innovative and creative thinking, deep thought, problem solving and independence more than the stem subjects. while it is widely commented on that there is a need to reimagine the ways stem subjects are taught in schools by embracing a more interactive and innovative approach to appeal to more students, it should come as no surprise that some of the most reputable educational organizations in the world are already implementing changes to embrace stem. harvard, for example, announced in 2014 that they were increasing their computer science faculty by over 50% and has since introduced computer science as a core subject for all freshmen. the urgency of supporting stem subjects at a young age is becoming more relevant than ever, as adopting a keen interest in the curricula needs to happen early. however, beyond the age issue is one of gender.

let me know if you need more

if the hares very faster than they are now, and thus the lynx's hunting success rate drops because it will be harder to catch them, the rate that will change will be the pray-rate. the pray-rate will change because more hares will be able to survive, thus more individuals for reproduction, so the end result will be increasing population of hares.

the predator-rate will remain pretty much the same because there will be more than enough pray, but the hunting success rate is stagnant.

d we did this already