For the Hill Reaction, you used DCIP dye at a concentration of 1x10-4 M. Our lab technicians have stock solution at a concentration of 100mM. Explain how you would make 100mL of the DCIP for you to use in a Hill Reaction lab.

by diluting the DCIP dye stock solution 1000 times. It can be done by diluting 0.1 ml of the DCIP dye stock solution with 99.9 ml of distilled water

Explanation:

1 millimolar (mM) = 0.001 M

1.0 × 10⁻⁴  Molar (M) = 0.0001 M = 0.1 mM

Stock solution = 100 mM = 0.1 M

100 mM DCIP dye solution diluited 1000 times  >> 0.1 mM = 1 x 10⁻⁴ M