What would be the first threat if the mining company were to start mining in the national park?

''Mining" probably conjures several images. One familiar scene is of the old West, where prospectors blast the sides of mountains, tunnel through the earth, or pan at a river's edge for gold. Another is of environmental impacts of acid mine drainage from older mines that did not benefit from modern technology and management practices. The common view of mining is of environmental degradation. Few individuals outside the industry are aware of modern mining practices and associated business, environmental, and public policy issues (highlighted in Appendix A) or of how mining companies are responding to today's environmental challenges.

Explanation:

this protocol provides a detailed procedure for co-immunoprecipitation (co-ip) to identify protein–protein interactions.