g With respect to their 3' and 5' ends, the two polynucleotide chains of a double-stranded DNA molecule are:

They are antiparallel

Explanation:

They are antiparallel because one strand of 3' faces the end of 5' of another strand and 5' end of one strand faces 3' end of another strand. Which indicate that 5 phosphate group faces the end of 3 hydroxyl group and they are of opposite directions to each other because it has opposite polarity with each other .

b is the best answer for this question.

the dna molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole. power is turned on and dna fragments migrate through gel (towards the positive electrode).

explanation: