Road construction has isolated a small population of a beetle population from the main population. This newly isolated population has very different allele frequencies than the main population, most probably because

genetic drift

Explanation:

The newly isolated population of beetle would have very different allele frequencies than the main population because of genetic drift.

Genetic drift refers to a change in the frequencies of alleles in a population over generations brought about by pure chance. Genetic drift can cause a bottleneck or founder effect. A bottleneck effect is a temporary decrease in the genetic diversity of a population while the founder effect refers to a decrease in the genetic variability of a new population formed from a small number of individuals that get separated from a larger population.  

Hence, the newly formed population of beetles would have lower allele frequencies than the main population due to the founder effect brought about by genetic drift.

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thinking it would be d not too sure but i think it's right