Answer the following questions on genomics using the information you learned from the lectures. There can be multiple answers per question in this section. Partial credit will be applied and incorrect answers will be penalized such that there is a minimum possible score of O per question. Which of the following is needed for the Illumina short read sequencing that we discussed? A. dideoxynucleotides B. TAQ DNA polymerase C. deoxynucleotides D. deoxynucleotides with fluorescent tags E. dideoxynucleotides with fluorescent tags What would happen if you tried to do standard PCR with dideoxynucleotides and four primers? A. Amplification would fail after the first base is added. B. Amplification would be exponential C. Amplification would be quadratic D. Amplification would fail because TAQ would be unable to add the first base. E. Amplified products would have a defined start and undefined end What are the unique components of a dideoxynucleotide that are necessary for Sanger dideoxy sequencing? A. ribose B. triphosphate C. dideoxyribose D. a chemical fluorescent tag E. monophosphate Which of the following makes TAQ polymerase bad for PCR reactions to amplify and clone DNA? A. It is able to synthesize DNA in both the 3-5' and 5-3' directions, thus increasing the potential for confused reactions B. It is able to remain functional at the high temperatures required to denature DNA C. It requires the addition of dNTPs, minerals, or salts to do a PCR reaction D. It is easy to purify. E. It is sloppy and causes mutations of 23