A first messenger molecule activates a receptor that stimulates Enzyme A, which synthesizes 100 second messenger molecules. In turn, each second messenger activates only one molecule of Enzyme B, which once activated, phosphorylates 100 molecules of Enzyme C that each phosphorylate 75 molecules of Enzyme D. In the last step, each Enzyme D phosphorylates 50 transcription factors. How many transcription factors are phosphorylated in response to the first messenger binding to the receptor