And im trying to look it up but it dosent even help

Human-induced rapid environmental change (HIREC) poses a threat to the persistence of many species and populations through factors such as climate change, habitat loss or fragmentation, increased human harvesting, exposure to novel biotic (e.g., predators, competitors, pathogens) or abiotic (e.g., pollutants) stressors

Explanation:

then that particular organisms become extinct.

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.