On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25 respectively. You are supplied with a filter that selects a wavelength of 520 nm.
a. What is the limit of resolution of this microscope?
b. Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?
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On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1...
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