Point mutations resulting in a single amino acid substitution provided evidence that: Select one: A. The genetic code is a triplet code. B. The genetic code is overlapping. C. The genetic code is not overlapping. D. The genetic code is degenerate. E. tRNAs recognize codons.

A change or modification in the single nucleotide base of the genetic material is called a point mutation. It can be either addition, deletion or inversion.

The correct answer is:

Option C. The genetic code is not overlapping.

This can be explained as:

Therefore, point mutation tells that the genetic code is non-overlapping.

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C. The genetic code is not overlapping.

Explanation:

The genetic code is nonoverlapping because each nucleotide can be part of only one codon or 'codeword' during protein synthesis. Therefore, a particular point mutation in the nucleotide sequence can result in only one amino acid modification. A nonoverlapping genetic code indicates that the same letter (i.e., the same nucleotide) can not be used for two different codons. The non-overlapping nature of the genetic code was discovered by Tsugita and Fraenkel-Conrat (1960) by observing point mutations in the tobacco mosaic virus.

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