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Biology, 11.02.2021 17:20 calebhoover03

You design primers for colony PCR with one primer binding to your gRNA insert (20 bp), and 1 primer binding to a region of your plasmid ~430 base pairs away. Your plasmid vector is pML104, which is 11,240 base pairs. In colony PCR, a positive result (indicating a colony likely carries a pML104 plasmid with the gRNA inserted) would be a PCR product that is:

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You design primers for colony PCR with one primer binding to your gRNA insert (20 bp), and 1 primer...

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