Put the following 6 steps in order to indicate the overall experimental flow. a. Quantify extracted protein
b. Transform BL21(DE3) w/ pDHFR
c. Run purified protein on gel to verify by SDS-PAGE and Western Blot
d. Generate pDHFR expression vector
e. Induce DHFR protein synthesis in E. coli with IPTG
f. Purify DHRF protein by affinity chromatography (IMAC)

By water evaporation from the leaves is created a negative pressure at the top of the tree that promotes the ascension  of the water through channels that are called xylem. there are also two molecule forces that are involved: the intra-water molecules cohesion (water molecules stick to each other) and the adhesion when the water molecules stick to the channels they are running in. this is what enables water to go from the roots to the top of a tall tree.

The awnser would be d im pretty sure

Answerfalse;

The structure of prokaryote and eukaryote cellsduring the 1950s, scientists developed the concept that all organisms may be classified as prokaryotes or eukaryotes. the cells of all prokaryotes and eukaryotes possess two basic features: a plasma membrane, also called a cell membrane, and cytoplasm. however, the cells of prokaryotes are simpler than those of eukaryotes. for example, prokaryotic cells lack a nucleus, while eukaryotic cells have a nucleus. prokaryotic cells lack internal cellular bodies (organelles), while eukaryotic cells possess them. examples of prokaryotes are bacteria and archaea. examples of eukaryotes are protists, fungi, plants, and animals (everything except prokaryotes).