Biology, 11.02.2021 23:10 saren47

You are studying expression of your favorite gene, YFG1. You have cloned the entire gene including its promoter to a plasmid. In addition to the wild type, you have also made mutations to two different parts of the gene that you suspect are the promoter. Let's call these mutants Mut1 and Mut2. After transforming these 3 plasmids into separate cultures of cells, describe an assay you could use to measure the amount of RNA produced, and how you would know if Mut1 or Mut2 have mutations in the promoter. Show what ideal results would look like. I want details and specifics - this is not a question you can answer with 2-3 sentences. (recommend minimum 300 words)

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You are studying expression of your favorite gene, YFG1. You have cloned the entire gene including i...

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