Where do hiccups come from?

Hiccups are caused by involuntary contractions of your diaphragm — the muscle that separates your chest from your abdomen and plays an important role in breathing. This involuntary contraction causes your vocal cords to close very briefly, which produces the characteristic sound of a hiccup

in situ hybridization has been widely used for research applications, including clinical cytogenetics, gene mapping, tumor biology and studies of chromosome evolution.