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The dna in a cell’s nucleus encodes proteins that are eventually targeted to every membrane and compartment in the cell, as well as proteins that are targeted for secretion from the cell. for example, consider these two proteins: phosphofructokinase (pfk) is an enzyme that functions in the cytoplasm during glycolysis. insulin, a protein that regulates blood sugar levels, is secreted from specialized pancreatic cells. assume that you can track the cellular locations of these two proteins from the time that translation is complete until the proteins reach their final destinations.for each protein, identify its targeting pathway: the sequence of cellular locations in which the protein is found from when translation is complete until it reaches its final (functional) destination. (note that if an organelle is listed in a pathway, the location implied is inside the organelle, not in the membrane that surrounds the organelle.)

Rease is an enzyme used by plants to break down urea (a nitrogen-containing compound) into carbon dioxide and ammonia. urease urea > > > carbon dioxide and ammonia ammonia is broken down by plants into a nitrogen source plants need to grow. thus, plants could not use urea as a nitrogen source unless it was first converted to ammonia. in soybean plants there are two different kinds of urease, one produced in the seeds and the other produced in the leaves of the plant. three types of soybean plants were used in a set of experiments: normal soybeans and two mutant strains, one lacking the urease in the seeds only (strain 1) and one lacking urease in the leaves only (strain 2). experiment 1 separate areas in a field were planted with normal, strain 1, and strain 2 soybeans. all types of soybeans appeared to grow, flower, and produce seeds equally well. there were no externally detectable differences among the strains. experiment 2 small pieces of plant leaves of equal weight were obtained from each type of soybean plant and separately placed on media in culture dishes. tissue growing in this way will become an unorganized clump of cells referred to as callus. to provide a controlled nitrogen source, half the tissue samples of each type were placed on media containing urea, and the other half of the samples were placed on media containing ammonia. after 30 days, the weight gain for each of the callus samples was determined. results are shown in the table below.

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