A lawn of Pseudomonas aeruginosa was grown for 24 hours and then scraped completely. The mass of bacteria was suspended in 5ml of sterile saline. An aliquot of the suspension was measured using a Spectrophotometer. The absorbance or OD600 of the initial suspension was measured at 0.892.

The suspension was serially diluted. In each tube 1ml of the previous dilution was added to 9ml of saline. In the boxes above the tubes write the dilution of that tube as a ratio. The first one is done for you.

Explanation:

2.20 2.60 3.7 3.40

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure

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