You are performing site directed mutogenesis to test predictions about which residues are essential for protein function, which of each pair of Amino acid substitute listed below will you expect to disrupt protein structure the most. Explain 1) isoleucine replaced by alanine or phenylalanine
2) lycine replaced by glutamine or arginine
3) asparagine replaced by glutamate or glutamine
4) proline replaced by histidine or glycine

The table presents the average day and night temperatures in five cities. it also reveals whether a city receives substantial rainfall (wet climate) or little rainfall (dry climate). which city’s rocks are likeliest to experience frost wedging, and why? a) city a because the consistently subzero temperature would prevent water from melting b) city b because it is a wet region and the temperature fluctuates around the freezing point c) city c because it receives plenty of rain fall and the weather is moderately cool d) city d because the hot and dry weather would cause rocks to absorb water